Co-culture of fibroblasts and pancreatic ductal adenocarcinoma cells with mouse peripheral ganglia

The peripheral nervous system (PNS) orchestrates organ function during homeostasis and stress. Most cancers including pancreatic ductal adenocarcinoma (PDAC) are infiltrated by PNS neurons participating in their complex tumor microenvironment. Here, we analyze the effect of neuronal interactions with fibroblasts and cancer cells with dorsal root and celiac ganglia. To this end, both cell types were co-cultured with both peripheral ganglia and subjected to bulk RNA-Sequencing.