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Host proteins interacting with EV71-VP1 were identified by mass spectrometry

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EV71-VP1-HA was first transfected into HEK293T cells, treated with MG132 or DMSO 36 h later, and cells were collected 12 h later. Cell lysates were enriched by protein-G with HA antibody (Roche,catalog no. 11867423001), separated by SDS-page, and stained with Coomassie Brilliant blue. Finally, the proteins interacting with EV71-VP1 were obtained by mass spectrometry.

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2025-01-07
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