Activation of MAP3K7 in response to WNT

Endogenous MAP3K7 is phosphorylated in a CAMK2-dependent fashion upon co-expression of WNT5A or after stimulation of calcium signaling (Ishitani et al, 2003a). Despite evidence that co-precipitated MAP3K7 is phosphorylated in vitro in the presence of CAMK2, direct phosphorylation of MAP3K7 by CAMK2 using purified proteins has not been demonstrated (Ishitani et al, 2003a). The roles of polyubiquitination and accessory proteins TAB1, 2 or 3 in MAP3K7 activation have not been investigated in the context of non-canonical WNT signaling and are therefore omitted from this reaction (reviewed in Dai et al, 2012).

内源性MAP3K7在WNT5A共表达或钙信号刺激后，以CAMK2依赖的方式发生磷酸化（Ishitani等，2003a）。尽管有证据表明，在体外存在CAMK2的情况下，共同沉淀的MAP3K7被磷酸化，但尚未通过纯化蛋白直接证明CAMK2对MAP3K7的磷酸化作用（Ishitani等，2003a）。在非典型WNT信号传导的背景下，尚未研究多泛素化及其辅助蛋白TAB1、2或3在MAP3K7激活中的作用，因此这些反应中未包含（参见Dai等，2012年的综述）。