Fibroblast-Derived Thrombospondin 2 Shapes the Immune-Exclusionary Environment at Invasive Fronts in Colorectal Cancer [Visium

Fibrotic colorectal cancers (CRC) are characterized by immune exclusion driven by activated stroma, particularly by cancer-associated fibroblasts (CAFs). This study aims to describe characteristics of spatial immune landscape of CRCs, which are subtyped by transcriptomic signatures and microsatellite instability. Overall design: Four slides from FFPE blocks of surgically resected CRC specimens, which were determined as CMS1/MSI, CMS2, or CMS4 using bulk-RNA-seq data, were analyzed using spatial transcriptomics (ST).