Identification, visualization and clonal analysis of intestinal stem cells in fish

Movie 1: The rostral
part of the medaka intestinal tract.

A 3D view
of X-ray data starting from the buccal cavity and going
through the mid-gut reveals distinct topographical regions along the rostro-­‐caudal axis.

Movie 2: The caudal
part of the medaka intestinal tract.

A 3D view
of X-ray data from the hind-gut indicating the narrower passage in the caudal
part of the intestine with smaller folds.

Movie 3: The GaudíRSGubiquitin:ERT2Cre (10dpi)

MuVi-Spim 3D visualization of a gut
segment at 10days after induction shows already labeling of strings of clonally
related groups representing the stem cells and their descendants (green).

Movie 4: The GaudíRSGubiquitin:ERT2Cre (30dpi)

MuVi-Spim 3D visualization of a gut
segment at 30 days after induction showing intestinal folds that are either
broadly labelled by H2B-­‐ EGFP (green), or not at all
(grey) indicating longitudinal
expansion of the clones.

Movie 5: The GaudíRSGubiquitin:ERT2Cre (150dpi)

 MuVi-Spim
3D visualization of a gut segment at 150 days after induction. Two large clones
(in green) established by symmetric division and neutral drift.

Movie 6:  The GaudíLxBBW hsp70:nlsCRE (90dpi)

MuVi-Spim 3D visualization of a gut segment
90 days after induction shows that stochastically triggered clonal multi-color
labeling results in adjacent clones of different sizes discernible by different fluorescent proteins demonstrated
in this movie by yellow, green, blue and purple color.