Supplementary datasets for: Prodigious polyphyly in Pleuroceridae (Gastropoda: Cerithioidea)

Phylogenomic studies with hundreds or thousands of loci are rare for most
invertebrate groups, including freshwater gastropods. This can prevent
understanding of phylogeny, which hinders many areas of research.
Pleuroceridae is a family of freshwater snails that is highly imperiled
and plays an essential role in the ecology of many freshwater systems of
the eastern United States. However, the evolutionary history of the family
is not understood, and the systematics of the family has not been revised
in a modern framework. Pleurocerids display a variety of egg-deposition
behaviors and shell shapes, making the family an ideal system for studying
evolution of invertebrate life history and morphology. However, past
mitochondrial-based phylogenetic analyses have failed to produce
meaningful phylogenetic hypotheses, preventing conclusions about
pleurocerid systematics and evolution. Here, we generated a novel anchored
hybrid enrichment probe set with phylogenetic utility for Pleuroceridae.
We sampled pleurocerids from across their range to test the probe set and
generated a backbone phylogeny. Our analyses uncovered striking levels of
polyphyly among currently accepted genera. Numerous species were also
polyphyletic, indicative of unrecognized diversity. Phylogenetic patterns
also revealed considerable convergence of shell morphologies. In contrast,
anatomical and life history features appeared to be much less homoplastic.
Despite generic paraphyly, high support for most major clades and
phylogenetic cohesiveness of non-shell characters indicate utility of the
AHE probe set for studying pleurocerid evolution.