Single-Cell RNA Sequencing Reveals Regional Reprogramming during Adaptation to Massive Small Bowel Resection in Mice

Single-cell RNA sequencing reveals interactions between the retinoid metabolism pathway and molecular “proximalization”, or regional reprogramming, of distal small intestine epithelium following proximal small bowel resection. This provides novel insight into physiological adaptation to short gut syndrome. Overall design: Single-cell RNA sequencing was performed on epithelial cells isolated from distal SI (ileum) of mice following small bowel resection vs. sham surgery.