Transcription profiling of IL-4-expressing CD4 T cells in the lung and lung-draining lymph nodes. Transcription profiling of IL-4-expressing CD4 T cells in the lung and lung-draining lymph nodes

Using single cell RNA-sequencing and whole-genome targeted transcription profiling, this study reveals that helminth infection causes the expansion of distinct but clonally related IL-4-competent CD4+ T cells in the lung and lung-draining lymphoid tissues providing insight into tissue-specific mechanisms of type-2 cytokine regulation and the lineage commitment of T-helper 2 (Th2) and T follicular helper (Tfh) cells. Overall design: Genome-wide targeted sequencing to profile transcription of 4 distinct CD4+ T cells in lymphoid and nonlymphoid tissues