Transcriptomic and metabolomic analysis of liver of black seabream (Acanthopagrus schlegelii) fed with Saccharina japonica supplmentation

Macroalgae Saccharina japonica (SJ) has been reported to reduce hepatic lipid accumulation (HLA) in mammals, thus, we hypothesized that dietary SJ can reduce HLA in fish. Firstly, we obtained the transcriptome of the fish by next generation sequencing (NGS) and de novo assbemby. We annotated the unigene of the transcriptome and analyzed differentially expressed genes (DEG) in liver of fish fed with 0, 2.5 and 10% SJ supplementation. Secondly, we identified the metabolite of metabolome by LC/MS analysis and analyzed differentially expressed metabolites (DEM) in liver of fish fed with 0, 2.5 and 10% SJ supplementation. Combined transcriptomic and metabolomic analysis revealed that several important DEG, DEM and co-enriched KEGG pathway were identified to be potentially involved in reducing hepatic lipid accumulation of fish.
File S1: DEG in liver of fish between treatments;
File S2: DEM in liver of fish between treatments.

日本海带（Saccharina japonica，简称SJ）据报告可降低哺乳动物的肝脂积累（Hepatic Lipid Accumulation，简称HLA），因此，我们提出了假设：膳食中添加SJ可能减少鱼类中的肝脂积累。首先，我们通过下一代测序（Next Generation Sequencing，简称NGS）和从头组装（de novo assembly）获得了鱼类的转录组。我们对转录组的单基因进行了注释，并分析了以0%、2.5%和10% SJ补充剂喂养的鱼类肝脏中的差异表达基因（Differentially Expressed Genes，简称DEG）。其次，我们通过液相色谱-质谱联用（LC/MS）分析鉴定了代谢组中的代谢物，并分析了以0%、2.5%和10% SJ补充剂喂养的鱼类肝脏中的差异表达代谢物（Differentially Expressed Metabolites，简称DEM）。转录组与代谢组的联合分析揭示了多个关键的DEG、DEM以及共富集的KEGG通路，这些通路可能参与减少鱼类肝脂积累的过程。

附件S1：处理组间鱼类肝脏的差异表达基因；

附件S2：处理组间鱼类肝脏的差异表达代谢物。