Next Generation Sequencing Quantitative Analysis of Wild Type and Mki67-/- 4T1 mouse mammary carcinoma cell histone modification

Ki-67 chromatin associated proteins that has been shown to broadly affect the transcription profiles of cells when knocked-out. To understand if those transcriptome changes are regulated by histone modifications we performed ChIP-seq analysis for three of the most recognized histone marks (H3K27me3, H3K4me3 and H3K27ac) as the regulators of gene expression, in wild-type (WT) and Ki-67 knockout (Mki67−/−) mouse 4T1 cells. ChIP-seq profiles for H3K27me3, H3K4me3 and H3K27ac histone marks of wild-type (WT) and two clones of Ki-67 knockout (Mki67−/−) mouse 4T1 cells, all in duplicate, were generated by deep sequencing using Illumina Hiseq2500.