Analysis of proteins immunoprecipitated with anti-FLAG antibodies from Leishmania donovani promastigotes expressing Cdc45-FLAG or carrying empty vector

This study was carried out to identify the interacting partners of Leishmania donovani Cdc45, a protein that is conserved in eukaryotic DNA replication. Accordingly, Cdc45 was expressed in Leishmania promastigotes with a C terminal FLAG tag and whole cell lysates were isolated from these Ld1S transfectant promastigotes. Whole cell lysates were also isolated from promastigotes harbouring empty vector. These lysates were used in immunoprecipitation reactions with anti-FLAG antibodies.