Transcriptome and translatome analysis of constitutively overexpression of FXR1 and control THP1 cells

FXR1 overexpression in THP1 cells causes changes in polysome associated mRNAs. This leads to a distinct translatome in the cell that contributes to chemo- and immune- survival The experiment is comprised of 8 HuGene 2.0 ST arrays, profiling input RNA and sucrose-gradient-purified polysomal RNA obtained from leukemic cells from Vector control and FXR1 Overexpressing THP1 cells Total RNAs and RNAs associated with polysomes were purified from THP1 cells (control vector), THP1 cells (FXR1 overexpression) and hybridization on affymetrix microchips. Polysomes were isolated by fractionation on sucrose gradients. RNA was purified from either total cell lysate and or from only polysome fractions. Translatome change was analysed after normalizing mRNA levels on polysome to their level in total cell lysate.