DNA methylation analysis of human primed and naive pluripotent stem cells

Human pluripotent stem cells have two major pluripotent states, primed and naive, and the heterogeneity among cell lines in each pluripotent state remains a major unresolved problem. We showed that the overexpression of H1FOO-DD, which has a short expression period by fusing the destabilized domain to the maternal-specific linker histone H1FOO, together with OCT4, SOX2, KLF4 and LMYC in human somatic cells improves the quality of reprogramming to primed and naive pluripotency. Bisulphite converted DNA from the 48 samples were hybridised to the Illumina Infinium Human Methylation 450K or EPIC BeadChip Kit