Gene expression profiles of TRA2B4 or exon 2 overexpressing HCT116 cells

The human transformer 2β gene (TRA2B) encodes a ultraconserved region (uc.138) in exon 2. TRA2B4 RNA is the transcript that contains the whole exon 2. To investigate the molecular mechanism underlying the influence of uc.138 expression in colon cancer cells, we established stable cell lines that overexpressed full-length TRA2B4 (ex 1–ex 10) or TRA2B exon 2. In this study, microarray-based global expression analysis were investigated using uc.138 overexpressing HCT116 cells. To investigate the molecular mechanism underlying the influence of uc.138 expression in colon cancer cells, we generated stable cell lines that overexpressed full-length TRA2B4 (ex 1–ex 10) or TRA2B exon 2 using HCT116 cells. Total RNA was extracted from cells using a RNeasy kit (Qiagen) and purified RNAs were labeled using a Low RNA Input Liner Amplification Kit and a succinimide-containing fluorescent dye (Cy3)-CTP according to the manufacturer’s protocol (Agilent). Expression profiles were measured using a whole human genome microarray (SurePrint G3 Human; Agilent).