Isolates used in the validation of theVerticillium simplex PCR assays.

ATarget isolates served as positive controls for the respective species and lineage specific primer pairs. Strain numbers were compiled from Figure 3 and Figure S2.BNegative control isolates were selected to represent all species that differed from the respective target species by four or fewer substitutions at the more divergent primer site, or were randomly selected if all non-target species differed by more than four substitutions from the target species (Table S4). Strain numbers were compiled from Figure 3 and Figure S2.CPCR primer pair targeting this species was not used in any multiplex PCR assay and was thus also tested with the set of 26 isolates in Table 5 used for multiplex assay validation.