Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse cerebellar granule neurons (jrc_mus-granule-neurons-3)

<b>Sample</b>: Cultured mouse cerebellar granule neurons (progenitors) transiently expressing HP1 mEmerald / H3.3 SNAP (JF525) / Lamin B Halo(JF646)<b>Sample Description</b>: Near isotropic FIB-SEM imaging of primary mouse granular neurons to study chromatin domains and their reorganization during development. Six cultured cells imaged in this dataset.<b>Protocol</b>: High pressure freezing, freeze-substitution resin embedding with 2%<b> </b>OsO₄ 0.1% UA 3% H₂O in acetone; resin embedding in Eponate 12.<b>Contributions</b>: Sample provided by David Solecki (St. Jude Children’s Research Hospital), prepared for imaging by Nirmala Iyer (HHMI/Janelia), with imaging by C. Shan Xu (Yale), and post-processing by Gleb Shtengel, and Harald Hess(HHMI/Janelia).<b>Acquisition ID</b>: jrc_mus-granule-neurons-3<b>Voxel size (nm)</b>: 4 x 4 x 5.37 (X, Y, Z)<b>Data dimensions (µm)</b>:<b> </b>31.2 x 12.0 x 20.2 (X, Y, Z)<b>Imaging start date</b>: 2019-11-10<b>Imaging duration (days)</b>: 11<b>Landing energy (eV)</b>: 700<b>Imaging current (nA)</b>: 0.88<b>Scanning speed (MHz)</b>: 0.200<b>Dataset URL</b>: s3://janelia-cosem-datasets/jrc_mus-granule-neurons-3/jrc_mus-granule-neurons-3.zarr/recon-2/em/<b>Visualization Website</b>: https://openorganelle.janelia.org/datasets/jrc_mus-granule-neurons-3<b>Publication</b>: Hoffman et al., 2020; Xu et al., 2017; Xu et al., 2021