kcat-values for glucosylation of eEF1A ternary complex by Lgt's.
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Yeast eEF1A (yEF1A) and mouse eEF1A (mEF1A) were incubated for 10 min with an equimolar concentration of HPLC-purified Phe-tRNAPhe and GTPγS or GDPβS (50 µM) as indicated. Afterwards the complexes were subjected to in vitro glucosyltransferase reactions with UDP-[14C]glucose (10 µM) and 5 nM Lgt1, 2 and 3. The data were autoradiographically quantified using ImageQuant. Data are given as the mean (±SD) of triplicates of 5 min time points.
创建时间：
2015-12-02
