Cell layer specific AGO1-loaded sRNA from Arabidopsis thaliana dcl234 triple mutant

microRNAs (miRNA's) regulation target gene expression, often transcription factors and as such control entire transcriptional networks. This control is important for various developmental transitions and stress responses in a wide range of eukaryotic organisms. While miRNA-mediated gene regulation has been investigated over time (temporal) and in whole organs or tissues in multiple different organisms highlighting their importance, there has been a distinct lack of focus on spatial resolution of miRNA biology. Here we present at cell-type specific resolution, miRNA loading and miRNA action within the Arabidopsis root. Our results for the first time demonstrate the multiple novel modes of miRNA action and illustrate the widespread nature of miRNA movement, at a genome scale within a complex eukaryotic organ. Overall design: 5 samples examined corresponding to FLAG-AGO1-IP for plants expressing FLAG-AGO1 under 5 specific root cell layer promoter (columella/pACR4, stele/pSHR, endodermis/pSCR, cortex/pCO2 and epidermis/pWER)