Real-time quantitative PCR analysis of chondrogenic markers in iMPC with different treatments
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE140330
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Conventional TGF-β3 containing CM showed limited efficacy in promoting iMPCs chondrogenesis, which necessitated the development of a more optimal chondrogenic medium for iMPCs. Classic chondrogenic medium (CM) was compared with optimal chondrogenic medium (CM+BMP6), and basic meidum (BM) were served as control. qPCR gene expression profiling: chondrogenic markers of BM, CM and CM+BMP6 were compared with each other.Total RNA from each sample was used for gene expression analysis and normalized to RPL13A.
创建时间:
2020-01-14



