Transcriptome sequencing of Anoectochilus roxburghii

Anoectochilus roxburghii, a rare medicinal orchid, is severely threatened by gray mold, necessitating green control strategies. Our previous study showed B. velezensis promotes A. roxburghii growth and enhances its symbiotic fungus P. indica abundance, leading us to hypothesize their cross-kingdom consortium exerts synergistic disease suppression and growth promotion. Given unclear interaction mechanisms and underexplored application in high-value medicinal plants, we constructed and evaluated this consortium, which significantly promoted growth and reduced disease incidence by 41.3%. This ecologically complementary consortium provides new insights into plant-microbe-pathogen interactions and a promising disease management strategy, which can stabilize ecological benefits with tailored agronomic practices to support sustainable agriculture.