Single cell level analysis of the Arabidopsis root transcriptome and chromatin accessibility

The nuclei of Arabidopsis root seedlings were isolated then use to establish a single cell level resolution transcriptome (sNucRNA-seq) and profile of chromatin accessibility (sNucATAC-seq) applying 10X Genomics technologies. The nuclei of the root of 7 days-old Arabidopsis seedlings grown on agar MS medium were isolated. Upon isolation, the nuclei suspension was used to create sNucRNA-seq and sNucATAC-seq libraries. Five and two independent replicates were generated to perform these experiments., respectviely. The libraries were sequenced using high-throughput sequencing technologies. The data were analyzed using CellRanger, Seurat and Signac.