Microarray analysis of skeletal muscle in aged mice

Sarcopenia is the age-induced, progressive loss of skeletal muscle mass and function, which is accompanied by reduced muscle performance. Individuals with sarcopenia often become bedridden or dependent on a wheelchair, leading to decreased quality of life. In this study, to better understand changes in skeletal muscle during sarcopenia, we performed a microarray analysis of skeletal muscle in young (13-week-old) and aged (26-month-old) mice. The microarray data shows that expression of the enzymes related to glucose and polyamine metabolism were decreased in aged mice compared with young mice. For microarray analysis, RNA was isolated from the gastrocnemius skeletal muscle of young mice (13 weeks of age) and aged mice (26 months of age). Samples from young and aged mice (N = 3 for each group) were used.