Gene expression analysis on renal tubule epithelial cells, mIMCD3, with or without FGF23 stimulation.

FGF23 has been known to play an important role of calcium-phosphorus (Ca-P) metabolism. Recently, biological roles of FGF23 other than Ca-P metabolism were reported, such as commitment to myocardium enlargement and immunological roles in spleen. Thus, it was thought FGF23 might have roles other than Ca-P metabolism in renal tubule epithelial cells. So, we tried to identify new biological roles of FGF23 in those cells using DNA microarray analysis in mouse renal tubule epithelial cell line, mIMCD3, with FGF23 stimulation. As the results, expressions of 8 genes were upregulated and those of 18 genes were downregulated in mIMCD3 with FGF23 stimulation compared to those in mIMCD3 without FGF23 stimulation. Three out of 8 upregulated genes were protein coding genes and remaining 5 genes were non protein coding genes. Fifteen out of 18 downregulated genes were protein coding genes and remaining 3 genes were non protein coding genes. To identify new biological roles of FGF23 in renal tubule epithelial cells other than Ca-P metabolism, the gene expression analysis using DNA microarray was performed using total RNA from mouse renal tubule epithelial cell line, mIMCD3 stimulated with 10ng/mL of FGF23 for 4hours. We use total RNA from mIMCD3 without FGF23 stimulation as a control. The gene expressions were directly compared between with and without FGF23 stimulation on mIMCD3.