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Nanopore Transposon Insertion Identification

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https://figshare.com/articles/dataset/Nanopore_Transposon_Insertion_Identification/8795144
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资源简介:
Supplementary Information for a manuscript investigating the ability of using nanopore sequencing for identifying the sites of transposon insertions in bacterial genomes. Includes raw .ab1 files for sequencing to verify some transposon insertions and documents describing these insertions, a document describing the creation of all strains sequenced within the manuscript, a gel picture of genomic DNA prior to sequencing using a Rapid Kit as well as genomic DNA prior to sequencing using the LSK109 kit, a fasta file containing query sequences for identifying transposon insertions in genomes, a file showing coverage intervals for reads mapped back to the DBL494 genome after CRISPR-Cas enrichment and sequencing, a perl program used to pull a subset of reads of interest from a fasta file with input from blast outfmt-6,
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2019-09-10
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