ADK interacts with SnRK1 and SnRK1-KD in yeast cells.
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The indicated bait proteins were expressed as GAL4 DNA binding domain fusions, and the prey proteins as GAL4 activation domain fusions, in yeast Y190 cells. SnRK1 indicates the full-length protein and SnRK1-KD denotes the kinase domain. Chloramphenicol acetyl transferase (CAT) and p53 were negative controls. Interaction was indicated by cell growth on medium lacking histidine and containing 50 mM aminotriazole. As an additional indicator of interaction, colonies were monitored for LacZ activity (blue color) using a filter-lift assay.
创建时间：
2015-12-02
