Additional file 5 of Impaired SorLA maturation and trafficking as a new mechanism for SORL1 missense variants in Alzheimer disease

Additional file 5: Table S3: Sequences of guide RNAs, ssODNs and primer pairs used for CRISPR/Cas9 gene editing and clone screening. For each variant, the nucleotide change (as a function of the genomic position and SORL1 cDNA) and the resulting protein change are indicated, together with the sequences of the guide RNAs, the corresponding ssODNs and the Sanger sequencing primer pairs. On ssODN sequences, lowercases correspond to intronic sequences. The position of the variant is indicated in red and the corresponding nucleotide triplet is underlined. The synonymous variants introduced to preclude re-cutting by the Cas9 are indicated in green and the corresponding nucleotide triplet is underlined.