Supporting data for "Behavioral and mechanistic characterization of addictive properties of the classic anesthetic nitrous oxide and the anti-addictive effects of the natural compound cryptotanshinone"

This research is focused on the mechanisms under the addictive properties of Nitrous oxide. In this research, we used behavioral test (Conditioned place preference), molecular biological technique (PCR, Western blot, flow), imaging methods (IF and rs-fMRI) to explore the mechanism. Using these methods, the rewarding effects and molecular changes of in nitrous oxide treating mice and rats.

本研究聚焦于研究一氧化二氮成瘾特性的作用机制。在本研究中，我们运用行为测试（条件位置偏好）、分子生物学技术（PCR、Western blot、流式细胞术）以及成像方法（免疫荧光和静息态功能磁共振成像）来探究其作用机制。通过这些方法，我们探讨了经一氧化二氮处理的鼠类和鼠的奖赏效应及分子层面的变化。