Reverse phase protein arrays (RPPAs) (in vitro)

Relative protein levels for each sample were determined by interpolation of each dilution curves from the standard curve antibody slide. All the data points were normalized for protein loading and transformed to a linear value. Linear values were transformed to Log2 value and then median‐centered for hierarchical cluster analysis. In the study presented here, To determine the biological effects of Radiation on signaling pathways in GBM cells in vitro (U251 &U87), we profiled the modulation of phosphorylated and non-phosphorylated proteins using RPPA under different doses of radiation treatment (RT).