Developmental Programming: Differing impact of prenatal testosterone and prenatal bisphenol-A -treatment on hepatic methylome in female sheep

Steroid hormones play a crucial role in pregnancy and fetal development, influencing metabolism, inflammation, and immune responses. Excessive prenatal exposure to steroids or environmental chemicals like bisphenol A can disrupt offspring's metabolism. This study examines how prenatal exposure to testosterone and BPA affects the liver's DNA methylome, which is critical for long-term health. Overall design: Whole genome bisulfite sequencing of liver tissue from two cohorts of Suffolk sheep: one exposed prenatally with testosterone (5 treated and 5 controls), and the other prenatally exposed to BPA (4 treated and 4 controls).