Proteomic characterization and comparison of spermatozoa proteomes between ram (Ovis. aries) and buck (Capra. hircus) using a data-independent acquisition-mass spectrometry (DIA-MS)

Artificial insemination in small ruminants is most commonly performed using fresh semen due to the low fertility rates typically achieved with frozen spermatozoa. Usually, when developing and applying assisted reproductive technologies, sheep and goats are often lumped together as one specie. In order to optimize sperm cryopreservation protocols in sheep and goat, differences in sperm proteomics between ram and buck are necessary to detect, which may contribute to differences in sperm function and fertility.