Slow and fast cortical cholinergic arousal is reduced in a mouse model of focal seizures with impaired consciousness

Patients with focal temporal lobe seizures often experience loss of consciousness associated with cortical slow waves, like those in deep sleep. Previous work in rat models suggests that decreased subcortical arousal causes depressed cortical function during focal seizures. However, these studies were performed under light anesthesia, making it impossible to correlate conscious behavior with physiology. We show in an awake mouse model that electrically induced focal seizures in the hippocampus cause impaired behavioral responses to auditory stimuli, cortical slow waves, and reduced mean cortical high-frequency activity. Behavioral responses are related to cortical cholinergic release at two different timescales. Slow state-related decreases in acetylcholine correlate with overall impaired behavior during seizures. Fast phasic acetylcholine release is related to variable spared or impaired behavioral responses with each auditory stimulus. These findings establish a strong relationship be..., , , # Slow and fast cortical cholinergic arousal is reduced in a mouse model of focal seizures with impaired consciousness --- This dataset contains all data used in the production of the above-named publication. Specifically, it contains recording sessions of awake mice electrophysiological (LFP/MUA), behavioral, and fiberphotometry data used for figure production. Details are described in the above-named publication. Codes used to analyze these data and produce described results are found at [https://github.com/BlumenfeldLab/Sieu-et-al_2024](https://github.com/BlumenfeldLab/Sieu-et-al_2024) or [https://doi.org/10.5281/zenodo.13991910](https://doi.org/10.5281/zenodo.13991910) ## Description of the Data and File Structure There are two types of files: '.mat' and '.csv' files. ### '.mat' files corresponding to relevant Spike2 channels exported to MATLAB files. Channels description: '_contra*' = Contralateral Hippocampus LFP signal (unit: microvolt) '_control_time = marker for the star...