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Abrogation of WNT signaling by dominant-negative TCF4

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE28467
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To assess cellular changes upon abrogation of the WNT-signaling pathway, we induced expression of a dominant-negative T-cell factor 4 (TCF4). This showed a remarkable overlap with activation of the unfolded protein response (UTR) in the same colon cancer cell line. 500,000 cells were plated in a 10 cm2 plate, and the following day, treated for 20 hrs with doxycycline (Dox) or vehicle. Three independent biological replicates per treatment.
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2021-07-20
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