A S/T motif controls reversible oligomerization of the Hsp40 chaperone DNAJB6b through subtle reorganization of a β-sheet backboneUntitled Item

1. 15N_relaxation: This folder contains the relaxation and chemical shift data (as defined in the filenames) used in the fits to the 4 state model for WT and T142A in the corresponding folders. The R2_R1.txt file contains the values of R2 and R1 for the main state A. The fit4state.py file fits the data to the 4 state model (requires the numpy and lmift modules) (currently set up for WT fit) states.py contains the chemical shift, relaxation and exchange matrices for various models<br>2. csp_fits Contains the chemical shift titration data for WT (Figure S11). The concentration of total CTD added is noted in the filename and each file has the format atom1;atom2 chemShift_atom1(Hz) chemShift_atom2(Hz)<br>3. methyl_relaxation 1H and 13C methyl CPMG data. The data are separated in two datasets, termed 'B1' and 'Cterm' for residues in proximity to ST16, ST17 and those in the C-terminus respectively. 1H and 13C exchange induced chemical shifts are provided in the X_ExchShifts_*.txt files The CPMG 3kHz folder contains the 1H, 13C r2eff values at 3kHz cpmg field used to derive r relationships between R2a at 600 MHz and 800 MHz fitCPMG_CS_2state.py performs fits to a 2-state model (requires the numpy and lmift modules) and states.py contains the chemical shift, relaxation and exchange matrices<br>