Data for: The antidepressant fluoxetine alters mechanisms of pre- and post-copulatory sexual selection in the eastern mosquitofish (Gambusia holbrooki)

1. Supplementary methods

Gas chromatography−tandem mass spectrometry protocol for the analysis of trace levels of fluoxetine in water.

2. Supplementary tables

Table S1. Sperm quality parameters measured with Computer Assisted Sperm Analysis (CASA).

Table S2. Summary of statistical models.

Table S3. Sperm quality measures in control (0 ng/L; n = 51), low-fluoxetine-exposed (30 ng/L; n = 50) and high-fluoxetine-exposed (380 ng/L; n = 53) males. ANCOVA results presented are for the effect of fluoxetine treatment on each response variable.

3. Supplementary figure

Figure S1. Relationship between total sperm count (×106) and standard length (mm) of control (0 ng/L; n = 32), low-fluoxetine (30 ng/L, n = 29) and high-fluoxetine (380 ng/L, n = 29) males. In each panel, points indicate measurements for individual fish within the corresponding treatment group. Lines indicate the fitted relationship between standard length and sperm count for the focal treatment (black line) and, for reference, the other treatments (grey lines).