ROHAN-regulated root transcriptome in response to external ammonium supply

Rice (Oryza sativa L) favors ammonium as its major nitrogen source in paddy field, though the molecular mechanisms underlying its tolerance towards ammonium remain largely unknown. We identified a rice mutant, root hypersensitive to ammonium nitrogen (rohan) showing root hyper-sensitivity to ammonium. In order to unveil the mechanism underlying ROHAN-mediated root tolerance to ammonium, we performed RNA-seq analysis of root meristems of rohan mutants and the wild-type (9522) grown in the media supplied with or without 2.5mM ammonium.