Transcriptomic Profile of Neuroinflammation in Alzheimer’s Mouse Brain Treated With and Without Extracellular Vesicles. Transcriptomic Profile of Neuroinflammation in Alzheimer’s Mouse Brain Treated With and Without Extracellular Vesicles

Expression of 757 genes related to neuroinflammation was analyzed across 4 treatment groups of male mice to investigate the neuroprotective effects of either human neural stem cell (hNSC) or induced pluripotent stem cell microglial-like (iMGL) cell-derived extracellular vesicles (EVs) in 5xFAD mouse model of Alzheimer’s Disease (AD). Overall design: Samples from 18 male mice, ages 25-26 weeks at the time of perfusion, were analyzed using the commercially available nCounter® Mouse Neuroinflammation Panel from NanoString™, with 4-5 samples for each of the 4 treatment groups. The 5xFAD transgenic mouse line was selected for the rapid neuropathological progression of beta-amyloid plaque deposition and subsequent cognitive dysfunction. 5xFAD mice were randomly assigned to one of three AD treatment groups that would receive 4 weekly retroorbital injections of either: hNSC-derived EVs or iMGL-derived EVs at a dose concentration of 2.25E+07 EVs/50 ul injection; or vehicle consisting of phosphate buffered saline without calcium or magnesium (PBS -/-). C57BL/6J mice served as WT controls and also received 4 weekly retroorbital injections of vehicle.