Single-cell RNA sequencing identifies cells exhibiting sexually dimorphic gene expression in the brain of the mosquito, Aedes aegypti

In this study, 10x Chromium technology was applied to quantify transcripts from single-cell nuclei of adult male and female brain of Aedes aegypti, a medically important mosquito vector that transmits yellow fever, dengue, chikungunya, and Zika viruses to humans. Overall design: Single nuclei from male and female mosquitoes were used for 10x single cell 3' RNA-seq libraries, and the libraries were sequenced (20,000 per nucleus, paired-end single indexing reads) by Novaseq 6000.