File S1 - Accelerated Activation of SOCE Current in Myotubes from Two Mouse Models of Anesthetic- and Heat-Induced Sudden Death

Table S1. Times for 10%, 50%, and 90% activation of ISkCRAC at room temperature in C57Bl6, Y524S/+, and dCasq-null myotubes. Table S2. Times for 10%, 50%, and 90% activation of ISkCRAC at physiological temperature in C57Bl6, Y524S/+, and dCasq-null myotubes. Table S3. Effect of azumolene (Azum, 50 µM) on times for 10%, 50%, and 90% activation of ISkCRAC at in C57Bl6 and Y524S/+ myotubes. Figure S1. ISkCRAC current density and pharmacology. (A) Average (±SE) ISkCRAC current density recorded from WT C57Bl6 , Y524S/+, and dCasq-null myotubes at -80 mV in control (Cntl, filled bars) and after addition of 1 µM Gd3+ (open bars). Data for wild-type are taken from Yarotskyy and Dirksen (2012). Numbers of paired experiments are shown in bars. *p B) Average (±SE) ISkCRAC current density recorded from WT C57Bl6, Y524S/+ , and dCasq-null myotubes at -80 mV in control (Cntl, black bars) and after addition of either 100 µM (C57Bl6 and Y524S/+) or 10 µM (dCasq-null) 2-APB (open bars). Numbers of paired experiments are shown in bars. *p Figure S2. The effect of PT on ISkCRAC magnitude and activation rate in myotubes from WT C57Bl6, Y524S/+, and dCasq-null mice. (A) Average (±SE) time required for 10% (T10%), 50% (T50%), and 90% (T90%) activation of ISkCRAC in WT C57Bl6, Y524S/+, and dCasq-null myotubes. (B) Average (±SE) maximum rate of ISkCRAC activation in WT C57Bl6, Y524S/+, and dCasq-null myotubes. *p C) Average (±SE) peak ISkCRAC current density recorded at -80 mV in myotubes from WT, Y524S/+, and dCasq-null mice. (DOCX)