Data acquired from testing the effect of PAR1 inhibitors on Plasmodium falciparum-induced loss of endothelial cell barrier function

This dataset consists of 18 .csv files of data underlying the graphs and charts in figures 2-6 of the related publication (see final paragraph for details).
The files are as follows:-Combined analysis data for fig 2_TEER_includes normalisation.csv-Combined analysis data for fig5_TEER_includes normalisation.csv-Combined analysis data for fig6_TEER_includes normalisation.csv-Data for fig2A+B+C_TEER_EA cells+inhibitors+thrombin_3.csv-Data for fig2C_TEER_EA cells+inhibitors+thrombin_1.csv-Data for fig2C_TEER_EA cells+inhibitors+thrombin_2.csv-Data for fig3_flow cytometry HBMEC.csv-Data for fig4A_TEER_aPC+thrombin_all time points shown.csv-Data for fig4B_TEER_RBC+IE+lysate_all time points shown.csv-Data for fig5+6_TEER_lysate+thrombin_#04-2+#91-2.csv-Data for fig5+6_TEER_lysate+thrombin_#91-1.csv-Data for fig5+6_TEER_lysate+thrombin_E5555-1+#04-1.csv-Data for fig5+6_TEER_lysate+thrombin_E5555-2.csv-Data for fig5+6_TEER_lysate+thrombin_Vorapaxar-1.csv-Data for fig5+6_TEER_lysate+thrombin_Vorapaxar-2.csv-Data for fig6_TEER_lysate_Vorapaxar+E5555+#04+#91-1.csv-Data for fig6_TEER_lysate_Vorapaxar+E5555+#04+#91-2.csv-Data for fig6_TEER_lysate_Vorapaxar+E5555+#04+#91-3.csv
The related publication investigated the hypothesis that the Thrombin-PAR1 coagulation/inflammation axis plays a significant role in barrier loss in cerebral malaria (CM), and that adjunct treatments targeting this might alleviate mortality or post-CM neurological sequelae in CM. The authors used a co-culture of primary human brain microvascular endothelial cells (HBMEC) with Plasmodium falciparum material, recombinant PfEMP1 or lysates from infected erythrocytes (IE), and measured barrier function by Trans Endothelial Electrical Resistance (TEER). A selection of Protease-Activated Receptor-1 (PAR1) inhibitors was tested for their ability to reverse the P. falciparum and thrombin induced decrease in barrier function.
The TEER data is from an initial screen with 10 PAR1 inhibitors and the human umbilical vein endothelial cell line EA.hy926 followed by more detailed testing of 4 selected inhibitors and HBMEC. In addition, the effect of IE or IE lysate on EPCR expression on HBMEC was determined by flow cytometry.