Lactose operon sequences from: Nature reserves suppress evolutionary rescue from extinction

Escherichia coli K-12 GM4792 is incapable of utilizing lactose (lac-). However, when cultured in M9 medium containing both lactose and glucose as mixed carbon sources, lactose-utilizing reverse mutations (lac+) can be observed. We isolated and sequenced single clones of both lac- and lac+, and identified the molecular basis underlying the lactose utilization. , Lactose operon sequences from single clones that are incapable of utilizing lactose (lac-) and those with lactose-utilizing reverse mutations (lac+) were obtained by PCR and sequencing, and these sequences were subsequently aligned., , # Lactose operon sequences

[https://doi.org/10.5061/dryad.h70rxwdvb](https://doi.org/10.5061/dryad.h70rxwdvb)

## Description of the data and file structure

Escherichia coli K-12 GM4792 is unable to utilize lactose (lac-). However, when grown in an M9 medium containing lactose and glucose as mixed carbon sources, we can observe reverse mutations that allow the bacteria to utilize lactose (lac+). We isolated and sequenced single clones of both lac- and lac+ strains, and the alignment of these sequences is presented.

### Files and variables

#### File: Fragment(alignment).fas

**Description:** Fragmented sequences of lactose operon 

#### File: Full\_length(alignment).fas

**Description:** Full length sequence of lactose operon 

## Code/software

Any gene sequence alignment sofware such as  BioEdit and DNAman can be used to view the data.

## Access information

Other publicly accessible locations of the data:

* no

Data was derived from the following sources:

* no