Bulk transcriptome sequencing (bulkRNA-seq) of peripheral blood leukocytes (PBLs) in the bacterial meningitis (BM) of children

Purpose: Profiling the bulk transcriptomes of PBLs in sepsis-developed BM progression. Methods: The total RNA of PBLs from BM patients were extracted, and constructed into cDNA library. Raw data of mRNA profiles were sequenced by paired-end strategy. Gene-counts were generated through handling sequencing data with the combined workflow of UMI-tools, STAR, Subread package and Samtools. Results: Successfully acquirng multiple bulk-transcriptomic profiles of PBLs from BM patients in different sepsis conditions. Overall design: mRNA profiles of PBLs in onset (named SB1) or recovered (named SB2) conditions of sepsis during BM progression. *** For consistency with the publication (PMID 36119025), new data have been added (GSM8118572-GSM8118583, February 2024). The original data (GSM4974422-GSM4974434, December 2020) are not discussed in the above publication but they remain. Reason: GSM4974422-GSM4974434 are discussed in a third-party publication (https://link.springer.com/article/10.1007/s10528-023-10530-6 Fig.2B). For clarity, GSE163196's original Title/Summary/Overall design are in supplementary file December2020_README.***