Exploration of Next-Generation Sequencing for studies on the philogenetic relationships among the big clades the spider family Pholcidae (Arachnida: Araneae)

Due to the amount of genomic information that can be obtained with NGS, the objetive of the project is to explore the different procedures and techniques into the use of genomics, which will allow answering future questions and corroborating previous hypothesis found with traditional sequencing about the phylogenetic relationships and evolutionary history in the family Pholcidae. While previous molecular studies on pholcid relationships using traditional sequencing have provided considerable progress, NGS could provide information for up to 300 genes and provide more accurate phylogenetic hypothesis among the phylogenetic relationships the subfamilies of Pholcidae. We will use both methodologies in parallel because traditional DNA sequencing methods will allow us to get information faster and the utility of the NGS approach for Pholcidae phylogeny is still to be explored.