Inferring long-distance movements of insects using combined hydrogen isotope and genetic analyses: A case study of the African edible bush-cricket

Stable hydrogen (H) isotopes and mitochondrial COI sequence data can be used to investigate long-distance movements of species. We collected African bush-cricket (Ruspolia differens) specimens from Uganda, Kenya and Zambia from 2016 to 2020. We analysed the stable H isotope ratios from nymphal and adult wing chitin (specimens collected from 12 study regions). We used cytochrome c oxidase subunit I (COI) genetic sequences for population genetic analysis (specimens collected from 17 study regions). For our analyses, we recorded the specimen's sex, phase of development (nymph, non-swarming, or swarming), and collection date and region.

Methods
For detailed information on the methods, please consult our paper: Valtonen et al. Inferring long-distance movements of insects using combined hydrogen isotope and genetic analyses: a case study of the African edible bush-cricket. Ecological Entomology, https://doi.org/10.1111/een.13410.