Tape-assisted fabrication method for constructing PDMS membrane-containing culture devices with cyclic radial stretching stimulation

Advanced in vitro culture systems have emerged as alternatives to animal testing and traditional cell culture methods in biomedical research. Polydimethylsiloxane (PDMS) is frequently used in creating sophisticated culture devices due to its elastomeric properties, which allow mechanical stretching to simulate physiological movements in cell experiments. We introduce a straightforward method that utilizes three types of commercial tape—generic, magic, and masking—to fabricate PDMS membranes with microscale thicknesses (47.2 µm for generic, 58.1 µm for magic, and 89.37 µm for masking) in these devices. These membranes are shaped as the bases of culture wells and can perform cyclic radial movements controlled via a vacuum system. In experiments with A549 cells under three mechanical stimulation conditions, we analyzed transcriptional regulators responsive to external mechanical stimuli. Results indicated increased nuclear YAP/TAZ activity in both confluent and densely packed cells under c..., , , # Data from: Tape-assisted fabrication method for constructing PDMS membrane-containing culture devices with cyclic radial stretching stimulation

[https://doi.org/10.5061/dryad.jq2bvq8hd](https://doi.org/10.5061/dryad.jq2bvq8hd)

We have submitted our datasets: measured tape thicknesses (01Commercial tape thickness.xlsx), measured PDMS membrane thicknesses (02PDMS membrane thickness.xlsx), analyzed stains (03Strain.xlsx), analyzed curvature (04Curvature.xlsx), analyzed Pearson's coefficient of different cell densities(05Different cell density_Pearson's coefficient.xlsx), analyzed Pearson's coefficient of confluent cells (06Confluent cells_Pearson's coefficient.xlsx), analyzed Pearson's coefficient of dense cells (07Dense cells_Pearson's coefficient.xlsx), analysis of YAP/TAZ nuclear/cytoplasmic fluorescence intensity (08YAPTAZnuclei&cytoplasm_FluorescenceAnalysis.xlsx)

## Descriptions

01Commercial tape thickness

The thicknesses of three different commercial tapes (generic, magic...