HIV Gag is expressed in vivo in resting CD4+T cells.

The percentage of integrated HIV expressing HIV Gag was calculated by dividing the Gag positive cells per sorted cells (column 3) by the correction for live cells (column 4). We then divided this number by the integrated copies per PBMC (column 5) and multiplied by the RU5 per cell of the sorted Gag + resting CD4+T cells (and then multiplied by 100) to get the percent of integrated expressing Gag. This calculation assumes that all of the integrated HIV DNA is in the resting CD4+T cell compartment of the PBMC, which makes the percent expressed an underestimate.