Transcriptome analysis of arbuscular mycorrhizal and non-mycorrhizal roots in rice

Rice (Oryza sativa Nipponbare) wild type were used as plant materials for RNA-seq analysis. Germinated seeds were grown singly in 100 mL pots. The soil consists of 20 g (bottom layer) of Akadama soil (tuff loam) and 45 g (upper layer) of Kanuma soil (weathered volcanic lapillus), Ezo sand (small pumice) and Nippi soil (granular potting soil) mixture. No other nutrients were added except Nippi soil and water was supplied from the bottom by maintaining a water level up to 5 mm in depth. We used arbuscular mycorrhizal (AM) and non-mycorrhizal (NM) control plants. AM plants were inoculated with 500 spores R. irregularis DAOM197198. All plants were cultivated in growth chamber under 15 h light 9 h dark photoperiods for 15 days.