MIGen_ExS: BioImage Study

The BioImage Study (BioImage Study: A Clinical Study of Burden of Atherosclerotic Disease in an At-Risk Population, NCT00738725), is a prospective, observational study aimed at characterizing subclinical atherosclerosis in U.S. adults (55 to 80 years old) at risk for clinical atherosclerotic cardiovascular disease (PMID: 25790876). All exome sequencing was performed at the Broad Institute of Harvard and MIT; samples sequence capture was performed using Illumina's ICE Capture reagent and sequencing was performed on an Illumina HiSeq 2000 or 2500.]]>
We performed a nested case-control experiment for incident cardiovascular events. Cardiovascular events are defined as myocardial infarction, coronary revascularization, or stroke. We included participants who passed the following sample quality control measures: contamination < 5%, missingness < 5%, concordance with genome-wide array data when available, and Ti/Tv, total variants / depth, and F inbreeding coefficient outliers. We also excluded individuals who were genotypically found to be within three degrees of relatedness. Cases include those who developed cardiovascular events after a median follow-up of 2.7 years. We excluded individuals who had clinical cardiovascular disease at baseline examination. Controls were matched to each case in the matched nested case-control experiment. Controls were matched for age (+/- 2 years), sex, smoking status, and type 2 diabetes status.]]>