mRNA profiling after TAC/Sham surgery in NRON KO/wt mice

The long-non coding RNA NRON is enriched in cardiomyocytes where it localizes in the nucleus. Unexpectedly, NRON-overexpression in a transverse aortic constriction-induced hypertrophy mouse model exacerbated hypertrophy, whereas cardiomyocyte-specific NRON-deletion attenuated cardiac hypertrophy, thereby suggesting a novel regulatory role of NRON in the heart. To gain insight into molecular processes affected by NRON KO in cardiac hypertrophy, we performed RNA sequencing of heart samples from WT and NRON KO mice 6 weeks after Sham and TAC surgery. Global gene expression was compared between three groups: WT mice exposed to Sham surgery (Sham WT), WT mice exposed to TAC surgery (TAC WT) and NRON KO mice exposed to TAC surgery (TAC KO).