Figure 3. Bifidobacterium strains induce unique changes in local and systemic immune cell distribution and LN architecture.
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Figure 3. Bifidobacterium strains induce unique changes in local and systemic immune cell distribution and LN architecture. A) Experimental design. C57BL/6 mice treated with antibiotics for 6 days followed by oral gavage with B. pseudolongum ATCC25526 (ATCC), UMB-MBP-01 (MD), or PBS (control). Mice then treated with the immunosuppressant tacrolimus for the next two days. Tissues harvested 2 days after oral gavage of bacteria. Frozen MLN sections stained for B) CD11c+ DC, and C) F4/80+ MF. Small intestine stained for D) Foxp3+ Treg, and E) CD11c+ DC. LN stained for laminin a4 and laminin a5 with their ratio depicted for F) MLN CR, and G) PLN CR. MFI values normalized using the sum of mean, and categories compared using one-way ANOVA. * p value
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2022-12-07



