Results showing specificity of the 18 mRNA markers used in various body fluids or tissues by endpoint PCR singleplexes and real-time PCR.

For all markers consisting of 4–16 individuals(as described in 2.2.1) were analyzed. Three separate experiments were analyzed. Initial total RNA was 1 ng.
1. ++ means dCt<10, peak heights>4000 RFU; + means 10≤dCt≤20, peak height 500≤RFU≤4000;
(+) means 2023, peak height <100 RFU;
2. m = adult male; f = female;
3. (Percentage) indicated the percentage of individuals showed positive or negative results of the marker in the tested body fluids or tissues;
4. ‘+’ or ‘−’ means 100% positive or negative for that marker.